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KMID : 0378019720150090065
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New Medical Journal
1972 Volume.15 No. 9 p.65 ~ p.74
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The Effects of Hypoxic and Hyperoxic Conditions on the Blood Elemets and Various Organs
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Abstract
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To investigate the effects of hypoxic and hyperoxic conditions on the erythrocyte, erythropoietin activity and heart metabolism, this experiment was performed to the animals of 24 rabbits and about 300 mice.
Hypoxic condition was induced by flowing the gas mixtures which contain 8% oxygen and 92% nitrogen into a closed hyperbaric chamber, and hyperoxic condition by flowing the gas mixture containing 40% oxygen and 60% nitrogen for 5 hours per day during the period of 15 days respectively.
The flow rates of each gas mixtures were corrected by analyzing the oxygen content of outlet gases with micro-Scholander apparatus.
Blood samples were obtained from the ear vein with heparinized syringes at the end of one-week and two-week exposure for the detemination of RBC, hemoglobin, hematocrit and RBC fragility and also arterial blood serum was collected and stored in freezer for the measurement of erythropoietin activity. This serum was injected subcutaneously to the plethoric mice and determined
59Fe incorporation rate.
Oxygen consumption and lactic acid level of left ventricle excised from the heart of sacrificed rabbit were determined.
The results obtained were as follows:
1. The increase of RBC count was more prominent in hypoxic group than in hyperoxic group.
2. Hemoglobin and hematocrit were increased significantly in hypoxic group and decreased in hyper Toxic group.
3. MCV, MCHC and MCH were increased in hypoxic group and decreased in hyperoxic group representing macrocytic shift in hypoxia and microcytic shift in hyperoxia.
4. Erythrocyte fragility was decreased in hypoxic group and increased in hyperoxic group.
5. Erythropoietin activity was increased in hypoxic group significantly, and decreased in hyperoxic group.
6. Oxygen consumption arid lactic acid level were elevated in hypoxia and depressed in hyperoxic group.
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KEYWORD
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